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Image Search Results
Journal: PLoS ONE
Article Title: Milk Fat Globule-EGF Factor VIII Attenuates CNS Injury by Promoting Neural Stem Cell Proliferation and Migration after Cerebral Ischemia
doi: 10.1371/journal.pone.0122833
Figure Lengend Snippet: (A) BrdU (purple) and DAPI (blue) immunofluorescent staining of cultured adherent embryonic neural stem cells after 24 h BrdU exposure and treatment with a. PBS as vehicle, b. 20 ng/ml rhEGF as positive control and increasing doses of rmMFG-E8; c. 125 ng/ml, d. 250 ng/ml and e. 500 ng/ml. Quantification of the number of proliferating adherent embryonic stem cells (BrdU + ) showed that rmMFG-E8 increased embryonic neural stem cell proliferation in a dose-dependent manner compared with PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; #p<0.05 vs rhEGF; n = 3/group. (B) rhEGF (20 ng/ml) and rmMFG-E8 (500 ng/ml) increased neurosphere formation compared to PBS. Data are presented as mean ± SEM. *p<0.05 vs PBS; n = 3/group. Scale bar = 100 μm. (C) rmMFG-E8 treatment showed significant increase in the number of neurospheres in the <0.5 mm size categories compared to PBS. *p<0.05 vs PBS; n = 3/group.
Article Snippet: Embryonic day-14
Techniques: Staining, Cell Culture, Positive Control